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Microparticle-enhanced nephelometric immunoassay of alpha-lactalbumin in human milk


Cuilliere, ML;Abbadi, M;Mole, C;Montagne, P;Bene, MC;Faure, G
JOURNAL OF IMMUNOASSAY
18: (1) 97-109 1997

Abstract:


A microparticle-enhanced nephelometric immunoassay was developed for alpha-lactalbumin quantitation in human milk. It is based on the nephelometric measurement of the light scattered during the competitive immunoagglutination of a microparticle-alpha-lactalbumin conjugate with an anti-alpha-lactalbumin antiserum.

This immunoassay is sensitive (detection limit in reaction mixture, 1.5 mu g/L) and could be performed in high dilution of milk, excluding any interference or sample pretreatment. It allowed the quantification of alpha-lactalbumin on a large range of concentrations (0.5-16.9 g/L) with accuracy (linear recovery in dilution-overloading assay) and precision (within- and between-run coefficients of variation from 1 to 7%).

Changes in the alpha-lactalbumin concentration of human milk during lactation were determined in 162 samples. The concentration and ratio of alpha-lactalbumin total protein were found to be significantly lower in colostrum (4.9 g/l, 27%) than in transitional milk (5.2 g/L, 40%), then decreased in mature milk (3.4 g/L, 31%).


Author Keywords:
alpha-lactalbumin, human milk, lactation, immunoassay, immunonephelometry, microparticle.

Addresses:
FAC MED VANDOEUVRE NANCY, IMMUNOL LAB, GRIP, F-54505 VANDOEUVRE NANCY, FRANCE.


Authors' E-mail Addresses:


Publisher:
MARCEL DEKKER INC, NEW YORK

TGA Number:
WH353

ISSN:
0197-1522